Publications

Aizawa H, Yanagihara S, Kobayashi M, Niisato K, Takekawa T, Harukuni R, McHugh TJ, Fukai T, Isomura Y, Okamoto H. (2013) The synchronous activity of lateral habenular neurons is essential for regulating hippocampal theta oscillation. J Neurosci. 33:8909-8921. PubMed Link
Okamoto H, Aizawa H. (2013) Fear and anxiety regulation by conserved affective circuits. Neuron 78:411-413. Review. PubMed Link
Aoki T, Kinoshita M, Aoki R, Agetsuma M, Aizawa H, Yamazaki M, Takahoko M, Amo R, Arata A, Higashijima SI, Tsuboi T, Okamoto H. (2013) Imaging of Neural Ensemble for the Retrieval of a Learned Behavioral Program. Neuron [Epub ahead of print] PubMed Link
Ohata S., Aoki R., Kinoshita S., Yamaguchi M., Tsuruoka-Kinoshita S., Tanaka H., Wada H., Watabe S., Tsuboi T., Masai I. and Okamoto H. (2011) Dual Roles of Notch in Regulation of Apically Restricted Mitosis and Apicobasal Polarity of Neuroepithelial Cells. Neuron. 69(2): 215-230. PubMed Link
Tanaka H, Nojima Y, Shoji W, Sato M, Nakayama R, Ohshima T, Okamoto H. (2011) Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons. Dev Dyn. 240:9-22. PubMed Link
Agetsuma M, Aizawa H, Aoki T, Nakayama R, Takahoko M, Goto M, Sassa T, Amo R, Shiraki T, Kawakami K, Hosoya T, Higashijima S, Okamoto H. (2010) The habenula is crucial for experience-dependent modification of fear responses in zebrafish. Nat Neurosci. 13:1354-1356. PubMed Link
Palmesino E, Rousso DL, Kao TJ, Klar A, Laufer E, Uemura O, Okamoto H, Novitch BG, Kania A. (2010) Foxp1 and lhx1 coordinate motor neuron migration with axon trajectory choice by gating Reelin signalling. PLoS Biol. 2010 Aug 10;8(8):e1000446. PubMed Link
Okamoto H, Ishioka A. (2010) Zebrafish research in Japan and the National BioResource Project. Exp Anim. 59:9-12. PubMed Link
Ito Y, Tanaka H, Okamoto H, Ohshima T. (2010) Characterization of neural stem cells and their progeny in the adult zebrafish optic tectum. Dev Biol. 342:26-38. PubMed Link
Drinjakovic J., Jung H., Campbell D.S., Strochlic L., Dwivedy A. and Holt C.E. (2010) E3 ligase Nedd4 promotes axon branching by downregulating PTEN. Neuron 341--357 PubMed Link
Amo, R., Aizawa, H., Takahoko, M., Kobayashi, M., Takahashi, R., Aoki, T., and Okamoto, H. (2010) Identification of the zebrafish ventral habenula as a homologue of the mammalian lateral habenula. J. Neuroscience. 30:1566-1574. PubMed Link
Isomura Y., Harukuni R., Takekawa T., Aizawa H. and Fukai T. (2009) Microcircuitry coordination of cortical motor information in self-initiation of oluntary movements. Nat. Neurosci. 12, 12, 1586--1593. PubMed Link
Sugiyama M, Sakaue-Sawano A, Iimura T, Fukami K, Kitaguchi T, Kawakami K, Okamoto H, Higashijima SI, Miyawaki A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc Natl Acad Sci U S A. 106(49):20812-7. PubMed Link
Wada H, Okamoto H. (2009) Roles of noncanonical Wnt/PCP pathway genes in neuronal migration and neurulation in zebrafish. Zebrafish. 6:3-8. Review. PubMed Link
Wada H, Okamoto H. (2009) Roles of planar cell polarity pathway genes for neural migration and differentiation. Dev Growth Differ. 51:233-240. Review. PubMed Link
Miyasaka N, Morimoto K, Tsubokawa T, Higashijima S, Okamoto H, Yoshihara Y.
(2009) From the olfactory bulb to higher brain centers: genetic visualization of secondary olfactory pathways in zebrafish. J Neurosci. 29:4756-4767.
PubMed Link
Carreira-Barbosa F, Kajita M, Morel V, Wada H, Okamoto H, Martinez Arias A, Fujita Y, Wilson SW, Tada M.(2009) Flamingo regulates epiboly and convergence/extension movements through cell cohesive and signalling functions during zebrafish gastrulation. Development. 136:383-392. PubMed Link
Okamoto H. (2008) An interview with Hitoshi Okamoto, M.D., Ph.D. Interview by Stephen C. Ekker. Zebrafish. 5:155-158. PubMed Link
Nishiwaki Y, Komori A, Sagara H, Suzuki E, Manabe T, Hosoya T, Nojima Y, Wada H, Tanaka H, Okamoto H, Masai I. (2008) Mutation of cGMP phosphodiesterase 6alpha'-subunit gene causes progressive degeneration of cone photoreceptors in zebrafish. Mech Dev. 125:932-946. PubMed Link
Okamoto, H., Sato, T., and Aizawa H., (2008) Transgenic technology for visualization and manipulation of the neural circuits controlling behavior in zebrafish, Develop. Growth Differ 50 Suppl 1:S167-75. PubMed Link
Yamaguchi, M., Fujimoto-Tonou, N., Yohimura, Y., Kishi, T., Okamoto, H., and Masai, I. (2008) Mutation of DNA primase causes extensive apoptosis of retinal neurons through the activation of DNA damage checkpoint and tumor suppressor p53, Development, 135:1247-57. PubMed Link
Aoki, M., Kiyonari, H., nakamura H., and Okamoto H. (2008) R-spondin2 expression in the apical ectodermal ridge is essential for outgrowth and patterning in mouse limb development. Develop. Growth Differ. 50: 85–95 PubMed Link
Omata Y, Nojima Y, Nakayama S, Okamoto H, Nakamura H, Funahashi J. (2007) Role of Bone morphogenetic protein 4 in zebrafish semicircular canal development. Dev Growth Differ. 49:711-719. PubMed Link
Okamoto H. (2007) Yin-yang ways of controlling gene expression are now in our hands. ACS Chem Biol. 2007 2:646-648. PubMed Link
Sassa T., Aizawa H., and Okamoto H., (2007) Visualization of two distinct classes of dorsal commissural neurons by gad2 and zic1 promoter/enhancer elements in developing zebrafish revealed the neuronal connectivity related to the auditory and lateral line system, Dev. Dyn. 236:706-718. PubMed Link
Aoki, M., Mieda, M., Ikeda, T., Hamada, Y., Nakamura, H., and Okamoto, H. (2007) R-spondin3 is required for mouse placental development. Devel. Biol. 301:218-226. PubMed Link
Tanaka H, Maeda R, Shoji W, Wada H, Masai I, Shiraki T, Kobayashi M, Nakayama R, Okamoto H. (2007) Novel mutations affecting axon guidance in zebrafish and a role for plexin signalling in the guidance of trigeminal and facial nerve axons. Development. Sep;134(18):3259-69. PubMed Link
Sato T, Hamaoka T, Aizawa H, Hosoya T, Okamoto H. (2007) Genetic single-cell mosaic analysis implicates ephrinB2 reverse signaling in projections from the posterior tectum to the hindbrain in zebrafish. J. Neuroscience May16;27(20):5271-9. PubMed Link
Sassa T, Aizawa H, Okamoto H. (2007) Visualization of two distinct classes of neurons by gad2 and zic1 promoter/enhancer elements in the dorsal hindbrain of developing zebrafish reveals neuronal connectivity related to the auditory and lateral line systems. Developmental Dynamics. Mar;236(3):706-18. PubMed Link
Aizawa H, Goto M, Sato T, Okamoto H. (2007) Temporally regulated asymmetric neurogenesis causes left-right difference in the zebrafish habenular structures. Dev Cell. Jan;12(1):87-98. PubMed Link
Wada H, Tanaka H, Nakayama S, Iwasaki M, Okamoto H. (2006) Frizzled3a and Celsr2 function in the neuroepithellium to regulate migration of facial motor neurons in the developing zebrafish hindbrain. Development. Dec;133(23):4749-59.Epub2006 Nov1. PubMed Link
Ando H, Okamoto H. (2006) Efficient transfection strategy for the spatiotemporal control of gene expression in zebrafish. Mar Biotechnol(NY). May-Jun;8(3):295-303. Epub2006 Apr18. PubMed Link
Sato, T. Takahoko, M. Okamoto, H. (2006) HuC:Kaede, a useful tool to label neural morphologies in networks in vivo. Genesis. Mar;44(3):136-42. PubMed Link
Hirate. Y. and Okamoto. H.(2006) Canopy1, a Novel Regulator of FGF signaling around the Midbrain-Hindbrain in Boundary in Zebrafish. Current Biology, 16:421-427. PubMed Link
Yamaguchi, M., Tonou-Fujimori, N., Komori, A., Maeda, R., Nojima, Y., Li, H., Okamoto., H., Masai, I. (2005) Histone deacetylase 1 regulates retinal neurogenesis in zebrafish by suppressing Wnt and Notch signaling pathways, Development in press. PubMed Link
Li, Q., Shirabe, H., Thisse, C., Thisse, B., Okamoto, H., Masai,I., Kuwada, J.Y. (2005) Chemokine signaling guides axons within the retina in zebrafish, J. Neuroscience 25:1711-1717. PubMed Link
Masai, I., Yamaguchi, M., Tonou-Fujimori, N., Komori, A., Okamoto, H. (2005) Requirement of Hedgehog signalling for the cell-cycle exit of retinal progenitor cells in zebrafish. Development, 132:1539-1553.  PubMed Link
Kawakami, A., Nojima, Y., Toyoda, A., Takahoko, M., Satoh, M., Tanaka, H., Wada, H., Masai., I., Terasaki, H., Sakaki, Y., Takeda, H., and Okamoto, H. (2005) The zebrafish-secreted matrix protein You/Scube2 is implicated in long-range regulation of Hdgehog signaling. Current Biology, 15: 480-488. PubMed Link
Miyasaka, N., Sato, Y., Yeo, S-Y., Chien, C-B., Okamoto, H., and Yoshihara, Y. (2005) Robo2 mediates the formation of an initial axon scaffold essential for establishment of precise glomerular map in the zebrafish olfactory system. Development, 132:1283-93. PubMed Link
Aizawa, H., Bianco, I.H., Hamaoka, T., Miyashita, T., Uemura, O., Concha, M.L., Russell, C., Wilson, S.W., and Okamoto, H., (2005) Laterotopic Representation of Left-Right Information onto the Dorso-Ventral Axis of a Zebrafish Midbrain Target Nucleus, Current Biology, 15: 238-243,  PubMed Link
Uemura, O., Okada, Y., Ando, H., Guedj, M., Higashijima, S., Shimazaki, T., Chino, N., Okano, H., and Okamoto, H. (2005) Comparative functional genomics revealed conservation and diversification of three enhancers of the isl1 gene for motor and sensory neuron-specific expression, Dev. Biol.278:567-606. PubMed Link
Emoto, Y., Wada, H., Okamoto, H., Kudo A., and Imai, Y. (2005) Retinoic acid-metabolizing enzyme Cyp26a1 is essential for determining territories of hindbrain and spinal cord in zebrafish, Dev. Biol. 278:415-427. PubMed Link
Ando H, Furuta T, Okamoto H. (2004) Photo-mediated gene activation by using caged mRNA in zebrafish embryos. Methods Cell Biol. 77:159-71. PubMed Link
Hanaoka, R., Ohmori, Y., Uyemura, K., Hosoya, T., Hotta, Y., Shirao, T., and Okamoto H. (2004) Zebrafish gcmb is required for pharyngeal cartilage formation. Mech. Dev. 121: 1235–1247 PubMed Link
Yeo SY, Miyashita T, Fricke C, Little MH, Yamada T, Kuwada JY, Huh TL, Chien CB, Okamoto H. (2004)Involvement of Islet-2 in the Slit signaling for axonal branching and defasciculation of the sensory neurons in embryonic zebrafish. PubMed Link
Miyashita T, Yeo SY, Hirate Y, Segawa H, Wada H, Little MH, Yamada T, Takahashi N, Okamoto H. (2004) PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branching. Development. 131(15):3705-15. PubMed Link
Okamoto H, Hirate Y, Ando H. (2004) Systematic identification of factors in zebrafish regulating the early midbrain and cerebellar development by odered differential display and caged mRNA technology. Front Biosci. 9:93-9 PubMed Link
Masai I, Lele Z, Yamaguchi M, Komori A, Nakata A, Nishiwaki Y, Wada H, Tanaka H, Nojima Y, Hammerschmidt M, Wilson SW, Okamoto H.(2003)N-cadherin mediates retinal lamination, maintenance of forebrain compartments and patterning of retinal neurites. Development  130(11):2479-94. PubMed Link
Hutson LD, Jurynec MJ, Yeo SY, Okamoto H, Chien CB. (2003)Two divergent slit1 genes in zebrafish. Dev Dyn. 228(3):358-69.   PubMed Link
Ando H, Okamoto H.  (2003) Practical procedures for ectopic induction of gene expression in zebrafish embryos using Bhc-diazo-caged mRNA. Methods Cell Sci.;25(1-2):25-31.  PubMed Link
Hirate Y., Okamoto H., & Yamasu K. (2003) Structure of the zebrafish fasciclin I-related extracellular matrix protein (βig-h3) and its characteristic expression during embryogenesis. Gene Expr Patterns. 3:331-6. PubMed Link
Okamoto., H., Segawa, H., and Higashijima, S., (2003) Toward genetic dissection of motor neuron differentiation.  Aquatic Genome-Steps toward a great future., Shimizu, N., Aoki, T., Takashima, F., eds., Springer-Verlag, Tokyo  
Bingham S, Higashijima S, Okamoto H, Chandrasekhar A. (2002) The Zebrafish trilobite gene is essential for tangential migration of branchiomotor neurons. Dev. Biol. 2002 242:149-60. PubMed Link

Zeller J, Schneider V, Malayaman S, Higashijima S, Okamoto H, Gui J, Lin S, Granato M. (2002) Migration of zebrafish spinal motor nerves into the periphery requires multiple myotome-derived cues. Dev. Biol.  252:241-56  

PubMed Link
Hirate, Y., Mieda, M., Harada, T, Yamasu, K. and Okamoto, H. (2001) Identification of ephrin-A3 and novel genes specific to the midbrain-MHB in embryonic zebrafish by ordered differential display. Mech. Devel. 107: 83-96.   PubMed Link
Ando, H., Furuta, T., Tsien, R. Y. and Okamoto, H. (2001) Photo-mediated gene activation using caged RNA/DNA in zebrafish embryos. Nature Genetics, 28: 317 –325. PubMed Link
Mizuno T., Kawasaki M., Nakahira M., Kagamiyama H., Kikuchi Y., Okamoto H., Mori K., and Yoshihara Y. (2001) Molecular diversity in zebrafish ncam family: three members with different vase usage and distinct localization. Mol Cell Neurosci. 18:119-30.  PubMed Link
Yeo, S.-Y., Little, M. H., Yamada, T., Miyashita, T., Halloran M. C, Kuwada, J. Y., Huh T.-L., Okamoto, H. (2001) Overexpression of a Slit Homologue Impairs Convergent Extension of the Mesoderm and Causes Cyclopia in Embryonic Zebrafish. Dev. Biol. 230:1-17.   PubMed Link
Masai, I., Okamoto, H., and Wilson, S. W. (2000) Midline signals regulate retinal neurogenesis in zebrafish. Neuron, 27:251-263.   PubMed Link
Saito, T., Hascilowicz, T., Ohkido, I., Kikuchi, Y., Okamoto, H., Hayashi, S., Murakami, Y., and Matsufuji, S. (2000) Two zebrafish (Danio rerio) antizymes with expression and activities. Biochem. J. 345: 99-106. PubMed Link
Higashijima, S., Hotta, Y., and Okamoto, H. (2000) Visualization of cranial motor neurons in live transgenic zebrafish expressing GFP under the control of the Islet-1 promoter/enhancer. J. Neurosci. 20: 206-218.   PubMed Link
Feinstein, Y., Borrell, V., Garcia, C., Burstyn-Cohen, T., Tzarfaty, V., Frumkin, A., Nose, A., Okamoto, H., Higashijima, S., Soriano, E., and Klar, A (1999) F-spondin and mindin: two structurally and functionally related genes expressed in the hipocampus that promote outgrowth of embryonic hippocampal neurons. Development 126: 3637-3648. PubMed Link
Mieda, M., Kikuchi, Y., Hirate, Y., Aoki, M., and Okamoto, H. (1999) Compartmentalized expression of zebrafish ten-m3 and ten-m4, homologues of the Drosophilatenm/odd Oz gene, in the central nervous system . Mech. Devel. 67: 223-227.   PubMed Link
Izsvak, Z., Ivics, Z., Shimoda. N., Mohn, D., Okamoto, H., Hackett, P. B. (1999) Short inverted-repeat transposable elements in teleost fish and implications for a mechanism of their amplification. J. Mol. Evol. 48: 13-21.   PubMed Link
Kikuchi, Y., Segawa, H., Tokumoto, Y., Hotta, Y., Uyemura, K., and Okamoto, H. (1999) Islet-3 in the regional specification of the brain. pp108-114, in Neural Development, Uyemura, K., Kawakami, K. and Yazaki eds., Springer.
Higashijima, S., Okamoto, H., Ueno, N., Hotta, Y., and Eguchi, G. (1997) High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoter of zebrafish origin. Dev. Biol. 192, 289-299.  PubMed Link
Higashijima, S., Nose, A., Eguchi, G., Hotta, Y., and Okamoto, H. (1997) Mindin/F-spondin family: novel ECM proteins expressed in the zebrafish embryonic axis. Dev. Biol. 192, 211-227.   PubMed Link
Kikuchi, Y., Segawa, H., Tokumoto, M., Tsubokawa, T., Hotta, Y., Uyemura, K., and Okamoto, H. (1997) Functional analysis of Islet-3 LIM/homeodomain protein in zrbrafish brain development. pp73-87, in Molecular Basis of Axon Growth and Nerve Pattern Formatoin (Taniguchi Symposium on Brain Science No. 20) Fijisawa, H. ed.  Japan Scientific Societies Press, Tokyo
Kikuchi, Y., Segawa, H., Tokumoto, M., Tsubokawa, T., Hotta, Y., Uyemura, K., and Okamoto, H.(1997).  Ocular and cerebellar defects in zebrafish induced by overexpression of the LIM domain of the Islet-3 LIM/homeodomain protein.  Neuron, 18, 369-382.   PubMed Link
Kim, C-H., Taira, E., Kuo, C-H., Li, B-S., Okamoto, H., Nakahira, K., Ikenaka, K., Higuchi, H. and Miki, N. (1996). Neuron-specific expression of a chicken gicerin cDNA in transient transgenic zebrafish. Neurochem. Res. 22, 231-137.   PubMed Link
Shimoda, N., Chevrette, M., Ekker, M., Kikuchi, Y., Hotta, Y., and Okamoto, H. (1996). Mermaid, a family of short interspersed repetitive element, is useful for the zebrafish genome mapping. Biochem. Biophys. Res. Comm. 220, 233-237.   PubMed Link
Shimoda, N., Chevrette, M., Ekker, M., Kikuchi, Y., Hotta, Y,. and Okamoto, H. (1996). Mermaid: a family of short interspersed repetitive elements widespread in vertebrates. Biochem. Biophys. Res. Comm. 220, 226-232.
Okamoto, H., Takahashi, M., Inoue, A., Gong, Z., Tsubokawa, T., Hew, C., Uyemura, K., and Hotta, Y. (1996). Isl-1 family in motoneuron specification and target recognition in embryonic zebrafish. pp401-412 in Basic Neuroscience in Invertebrate, Business Center for Academic Societies Japan, Ed. H. Koike.
Tokumoto, M., Gong,Z., Tsubokawa, T., Hew,C., Hotta, Y. Uyemura,K., and Okamoto, H. (1995). Molecular heterogeneity among primary moto-neurons and within myotomes revealed by differential mRNA expression of novel Isl-1 homologs in embryonic zebrafish. Dev. Biol. 171, 578-589.  PubMed Link
Kuo, C.-H., Uetsuki, T., Kim, C.-H., Tanaka, H., Li, B.-S., Taira, E., Higuchi, H., Okamoto, H., Yoshikawa, K., and Miki, N., (1995). Determi-nation of a necidin cis-acting element required for neuron specific expression by using zebrafish. Biochem. Biophys. Res. Comm. 211, 438-446.   PubMed Link
Inoue, A., Takahashi, M., Hatta, K., Hotta, Y., and Okamoto, H. (1994). Developmental regulation of Isl-1 mRNA expression during neuronal differentation in embryonic zebrafish. Devel. Dynam, 199, 1-11. PubMed Link
Sakai, Y., Okamoto, H., Mogami, K., Matsuo, H., and Hotta, Y. (1991). Heat  shock gene activation by mutant actin is independent of myofibril degeneration in Drosophila muscle. J. Biochem. 109, 670-673.
Okamoto, H., and Kuwada, J. Y. (1991). Alteration of pectoral fin nerves following ablation of fin buds and by ectopic fin buds in the Japanese Medaka fish. Dev. Biol. 146, 62-71.
Okamoto, H., and Kuwada, J. Y. (1991). Outgrowth by fin motor axons in wild type and a finless mutant of the Japanese Medaka fish. Dev. Biol. 146, 49-61.
Sakai, Y., Okamoto, H., Mogami, K. Yamada, T., and Hotta, Y. (1990). Actin with tumor related mutation is antimorphic in Drosophila muscle: Two distinct modes of myofibrillar disruption by antimorphic actins. J. Biochem. 107, 499-505.
Kuwada, J. Y., Okamoto, H., Bernhardt, R., and Chitnis, A. J. (1990). Growth cone guidance in a simple vertebrate nervous system. pp5-16. in Advance in Neural Regeneration Research. Wiley-Liss,Inc.
Sakai, K., Okamoto. H., and Hotta, Y. (1989). Pharmacological chara-cterization of sodium channels in the primary culture of individual Drosophila embryos: neurons of a mutant deficient in a putative sodium channel gene. Cell Differentiation and Development (Mech. Devel.) 26, 107-118.
Yoshihara, M. Takasu-Ishikawa, E., Sakai, Y., Hotta, Y., and Okamoto, H. (1988). Single P-element  insertional mutagenesis in Drosophila melenogaster. Proceedings of the Japan Academy. 64, SerB, 172-176.
Okamoto, H., Sakai, K., Goto, S., Takasu-Ishikawa, E., and Hotta, Y. (1987) . Isolation of Drosophila genomic clones homologous to the eel sodium channel gene. Proceedings of the Japan Academy. 63, SerB, 284-288. 
Okamoto, H., Hiromi. Y., Ishikawa, E., Yamada. T., Isoda, K., Maekawa, H., and Hotta, Y. (1986).  Molecular characterization of mutant actin genes which induce heat-shock proteins in Drosophila flight muscles. EMBO J.  5, 589-596.
Hiromi, Y., Okamoto, H., Gehring, W. J., and Hotta, Y. (1986). Germline transformation with Drosophila melanogaster mutant actin genes induces constitutive expression of heat shock genes. Cell  44, 293-301.
Okamoto, H., Hiromi, Y., Ishikawa,E., Yamada, T., and Hotta, Y. (1985). Molecular analyses of Drosophila melanogaster mutant actin genes. pp75-84. in Proceedings of the 11th International Symposium, Division of Biophysics, the Taniguchi Foundation.